1. Introduction

Fructosyltransferase, EC 2.4.1.9, which also called b- Fructo-furanosidase, EC 2.4.1.26, it is a kind of hydrolase with metastasis activity and wide receptor activity, which could be sucrose as raw material to synthesis kestose and other oligosaccharide. Fructo-oligosaccharide, also known as Fructooligosaccharide (FOS), is an excellent food ingredient, which has the physiological effect of probiotics. FOS is mainly contained Kestose, but also contains Nystose and 1F-fructofuranosylnystose.

We use the Aspergillus Niger high yield bacterium to refine after the deep fermentation. If we use this product to produce FOS, the conversion rate could achieve 56%-59%.

2. The reaction sugar spectrum of FOS synthesized by typical enzymatic method.

3. Characteristic

a.     Temperature: The best suitable temperature is 45-50℃

b.     pH: The best pH range is 5.0-5.5

c.     Effects of metal ions on enzyme activity: EDTA has an active effect on enzymes.

4. Specification

a.     Activity: 3000U/mL

b.     Enzyme activity Definition: In the best enzymatic reaction condition (45℃,ph5.5), converts sucrose into oligosaccharides, the amount of enzyme required to produce 1pmoL kestose per minute is defined as an enzyme activity unit (U).

5. Storage

Sealed and Kept in dry and cool place